Expression of annexin A9 in gastric cancer and its effect on proliferation of gastric cancer cell line SGC-7901 / 中华普通外科杂志

ABSTRACT

Objective:To investigate the expression of annexin A9(ANXA9)in gastric cancer tissues and cells and its effect on the proliferation of gastric cancer cells and the ability of subcutaneous tumorigenesis in nude mice.Methods:Immunohistochemistry and qPCR were used to detect the relationship between the expression of ANXA9 and clinicopathological parameters and prognosis in gastric cancer and paired adjacent tissues.Lentivirus transfection was used to inhibit the expression of ANXA9 in gastric cancer cell line SGC-7901. Cell counting kit-8 (CCK-8) and clone formation were used to detect the changes of proliferation of SGC7901, flow cytometry to detect the changes of SGC-7901 cell cycle, and nude mouse model bearing subcutaneous gastric cancer xenograft was established using SGC-7901 cells with stable ANXA9 knockdown to assess the effect of low expression of ANXA9 on xenograft growth.Results:Immunohistochemistry showed that the expression level of ANXA 9 was 67.1% and 30.7% in gastric cancer tissues and adjacent tissues, respectively. qPCR showed that the expression levels of ANXA 9 mRNA in gastric cancer tissues and adjacent tissues were 0.142±0.107 and 0.819±0.191, respectively. The difference was statistically significant ( P<0.05). The high expression of ANXA9 was different from the low expression group in the degree of tissue differentiation ( P<0.05), and the median survival time was 50 and 59 months, respectively. OD values of the transfected cells were 0.285±0.025, 0.386±0.031, 0.711±0.032, 1.007±0.084, 1.552±0.055 and 0.274±0.026, 0.380±0.049, 0.714±0.035, 1.106±0.081, 1.561±0.060, respectively, compared with 0.294±0.011, 0.445±0.046, 1.076±0.096, 1.588±0.095, 2.286±0.110 in NC group ( P<0.05). Cell clone formation in the transfection group was 207±12 and 225±14, lower than that in the NC group (412±14, P<0.05). After inhibiting the expression of ANXA9, the proportion of G 0/G 1 phase cells in the transfection group was 62.80% and 55.87%, respectively, significantly increased compared with 44.37% in the NC group. The proportions of S-phase cells in the transfected group were 22.74% and 21.44%, respectively, which were significantly lower than that in the NC group 29.19% ( P<0.05), after stable interference with ANXA9, the growth rate of transplanted tumors was significantly slower than that of the control group. On the 23rd day, the average volume of transplanted tumors in the two groups were (625±49) mm 3 and (303±157) mm 3, respectively, and the mass of tumor tissues in the two groups were (1.60±0.11) and (0.57±0.09) g ( P<0.05). Conclusions:Down-regulation of ANXA9 expression can inhibit the proliferation of gastric cancer cells and the ability of subcutaneous tumor formation in nude mice.