Hyperbaric oxygen inhalation downregulates the expression of BCL2-associated protein X and upregulates the expression of the B lymphoblastoma-2 gene after cerebral hemorrhage / 中华物理医学与康复杂志

ABSTRACT

Objective:To explore any effect of inhaling hyperbaric oxygen on edema resulting from cerebral hemorrhage.Methods:Cerebral hemorrhage was modeled using collagenase in 125 Wistar rats, with 5 others used as controls. The injured rats were randomly divided into a second control group without any hyperbaric oxygen intervention, and groups which inhaled hyperbaric oxygen for 40 min, 60 min, 80 min or 100 min. There were 25 rats in each group. The inhalation began 6 hours after the modeling and was administered once a day. On the 2nd, 4th, 6th, 8th and 16th day of the treatment, 5 rats from each group were sacrificed and the expression of the B lymphoblastoma-2 gene (Bcl-2) and BCL2-associated protein X (Bax) in the brain tissue around the hematoma was detected using immunohistochemical methods.Results:The average Bax expression in all of the HBO groups was significantly lower than in the second control group at each time point, but with significant differences between the HBO 60 min and HBO 40 min groups, as well as between the HBO 80 min and HBO 100 min groups. By the 4th day the average Bcl-2 expression in the HBO 40 min group was significantly different from that in the HBO 80 min group, and the same was true of the HBO 60 min and HBO 100 min groups.Conclusions:One of the mechanisms by which HBO treats intracerebral hemorrhage may be related to down-regulating the expression of Bax and increasing the expression of Bcl-2. The best everyday treatment duration is 80 min.