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Effect of virus inactivation on weak positive results of nucleic acid test for 2019 novel coronavirus / 中华检验医学杂志
Chinese Journal of Laboratory Medicine ; (12): 358-363, 2020.
Article in Chinese | WPRIM | ID: wpr-871924
ABSTRACT

Objective:

To investigate the effect of virus inactivation on weak positive result of 2019 novel coronavirus(2019-nCoV) nucleic acid test.

Methods:

A retrospective study was conducted on the nasopharyngeal swabs of three patients with positive PCR nucleic acid test for 2019-nCoV at different concentrations in the Second affiliated Hospital of Zhejiang University Medical College from January to February 2020.The virus in nasopharyngeal swab specimens were inactivated by water bath at 56 ℃ for 30 min, dry bath at 56 ℃ for 60 min and dry bath at 60 ℃ for 30 min respectively. After treatment, these samples RNA were extracted and then detected by three new commercial quantitative real-time polymerase chain reaction reagent kits for 2019-nCoV.Cycle threshold (Ct) value was used to evaluate the effect of virus inactivation on nucleic acid detection of 2019-nCoV.

Results:

There was no significant difference between the groups before and after inactivation. Ct values of ORF1ab gene before inactivation were 23.28±0.28, 25.25±0.25, 28.93±0.44, 32.06±0.47, 35.20±0.38, 32.89±0.38, 36.24±0.23, 33.30±0.46, and those after inactivation were, group 123.60±0.20, 27.29±0.30, 31.83±0.51, 37.41±0.46, group 2 24.25±0.34, 27.18±0.42, 31.84±0.61, 34.99±1.01, 34.89±0.45,group 3 23.37±0.17, 26.89±0.52, 32.05±0.50.Ct value of N gene before inactivation were 24.38±0.09, 26.64±0.11, 30.35±0.12, 33.29±0.33, 36.93±0.11, 34.50±0.12, 35.63±0.12, those after inactivation were, group 1 24.66±0.11, 28.52±0.14, 32.71±0.14, 37.00±0.13;group 2 25.41±0.10, 28.79±0.15, 33.29±0.28; group 3 23.37±0.11, 28.68±0.11, 33.54±0.13, 37.18±0.23(ORF1ab gene t=-1.416; N gene t=-1.379, P>0.05). There was no significant difference among the three inactivation groups, the specific Ct values are shown above(ORF1ab gene t=-0.460; N gene t=-0.132, P>0.05). However, the Ct values of the inactivated groups (1,2,3) and the non-inactivated group at different dilution times were different (10 ×Ct value of ORF1ab was 25.25±0.25 in the non-inactivated group, and 27.29±0.30, 27.18±0.42 and 26.89±0.52 in the inactivated group1,2 and 3, t(ORF1ab)=-7.327, P<0.01.Ct value of N gene in the non-inactivated group was26.64±0.11, those in inactivated group 1, 2 and 3 were 28.52±0.14, 28.79±0.15 and 28.68±0.11, respectively, t (N)=-19.340, P<0.01. 100 ×Ct value of ORF1ab was 28.93±0.44 in the non-inactivated group, and 31.83±0.51,31.84±0.61 and 32.05±0.50 in the inactivated group1,2 and 3, t (ORF1ab)=-9.462, P<0.01. Ct value of N gene in the non-inactivated group was 30.35±0.12, those in the inactivated group 1, 2 and 3 were 32.71±0.14, 33.29±0.28 and 33.54±0.13, respectively, t (N)=-18.583, P<0.01. The positive detection rate of the non-inactivated group (7/11, 8/11, 5/11) was significantly different from that of the inactivated group (inactivated group 14/11, 4/11, 3/11, inactivated group 23/11, 3/11, 3/11, and inactivated group 33/11, 3/11, 2/11) ( Z=-2.670, P<0.01). There were no significant difference among the inactivated groups(inactivated group 14/11, 4/11, 3/11, inactivated group 23/11, 3/11, 3/11, inactivated group 33/11, 3/11, 2/11) ( Z=4.413, P>0.05) and among the three reagents(reagent 17/11, 4/11, 3/11, 3/11, reagent 28/11, 4/11, 3/11, 3/11, reagent 35/11, 3/11, 3/11, 2/11)(χ 2=1.199, P>0.05).

Conclusion:

The virus inactivation can degrade the nucleic acid of the 2019-nCoV, resulting in the decrease of the Ct value and the false negative results of the low-concentration specimens.
Full text: Available Index: WPRIM (Western Pacific) Type of study: Observational study Language: Chinese Journal: Chinese Journal of Laboratory Medicine Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Observational study Language: Chinese Journal: Chinese Journal of Laboratory Medicine Year: 2020 Type: Article