Study on the NDM-1 carbapenemases producing Acinetobacter baylyi / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
;
(12): 587-590, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-871941
ABSTRACT
Objective:
To investigate the drug resistance genes carried on a carbapenem-resistant Acinetobacter spp. isolate.Methods:
A retrospective study was conducted on one strain of carbapenem-resistant Acinetobacter spp, which was clinically isolated in July 2016. The strain was identified by Vitek2-compact and further confirmed by PCR detection of bla OXA-51-like, 16S-23S rRNA intergenic space sequencing and partial rpoB gene sequence analysis. Carbapenemase (NDM, KPC, VIM, IMP, SIM, SPM, GIM, OXA-23-like, OXA-24-like, OXA-58-like), 16S rRNA methylase gene (armA, rmtA, rmtB), Beta-lactamases (TEM, SHV, CTX-M, VEB, PER) and class 1 integron were amplified and detected by PCR, and plasmids were analyzed using Southern hybridization.Results:
The Acinetobacter baylyi isolate showed the positive for bla NDM-1 only, and negative for the other genes. Plasmid analysis and Southern bybridization result revealed that the bla NDM-1 gene was located on unconjugatable plasmid (54 000-60 000 bp).Conclusions:
Attention should be paid to the occurrence of carbapenem-resistant non-baumannii Acinetobacter spp. and the transmission mechanism of drug resistant genes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Observational study
Language:
Chinese
Journal:
Chinese Journal of Laboratory Medicine
Year:
2020
Type:
Article
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