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Mechanism of Xiaoyaosan in Preventing and Treating Hepatic Injury Caused by Tripterygium Glycosides / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 76-82, 2020.
Article in Chinese | WPRIM | ID: wpr-872701
ABSTRACT

Objective:

To observe the preventive and therapeutic effects of 5-week administration with Xiaoyaosan on rat liver injury caused by tripterygium Glycosides.

Method:

Thirty-one SD rats were randomly divided into 4 groups, namely normal group, tripterygium glycosides group, tripterygium glycosides+Xiaoyaosan group (treatment group), and tripterygium glycosides+Xiaoyaosan for 1 week in advance group (prevention group). Tripterygium glycosides (37.5 mg·kg-1) was administered intragastrically, and Xiaoyaosan (water decoction, 19.270 g·kg-1) was administered intrastrically. First, the rats of prevention group were intragastrically administrated with Xiaoyaosan at 8:00-9:00 am, and the rats of other groups were given an equal volume of normal saline. After 1 week, the rats of tripterygium glycosides group were administered intragastrically with tripterygium glycosides suspension at 8:00-9:00 am. The rats of the treatment group and the prevention group were intragastrically administrated with Xiaoyaosan at 8:00-9:00 am, and then tripterygium glycosides suspension 2 hours later. All the drugs were given once a day for 5 weeks. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of liverenzyme-linked immunosorbent assayELISA) was used to detect serum interleukin-1β (IL-1β), interleukin-6IL-6) and tumor necrosis factor-α (TNF-α), chemical method was used to detect the content changes of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione peroxidase (GSH-Px), superoxide dismu-tase (SOD), trace malondiamine aldehyde (MDA). Immunohist-ochemical staining was used to observe the expressions of IL-6IL-1β and TNF-α in liver tissue.

Result:

Compared with the normal group, the tripterygium glycosides group showed inflammatory cell infiltration, hepatocyte edema, hepatic sinuses squeezing and narrowing, liver plate widening, and liver cell necrosis, the contents of serum IL-1β, IL-6, TNF-α, ALT, AST and MDA were significantly increased(P<0.01), but the contents of GSH-Px and SOD were decreased significantly (P<0.05, P<0.01), while the positive expressions of IL-6, TNF-α, IL-1β in liver tissue were significantly increased (P<0.01). Compared with tripterygium glycosides group, rats in the treatment group and the prevention group had less inflammatory cells infiltration and reduced edema in the liver tissue, and disorders in some cell, the contents of serum IL-1β, IL-6, TNF-α, ALT, AST, MDA were significantly decreased (P<0.01), the contents of GSH-Px and SOD were significantly increased (P<0.05), and the positive expressions of IL-6, TNF-α, IL-1β in liver tissues were significantly decreased (P<0.01), with a better efficacy in the prevention group.

Conclusion:

Xiaoyaosan can obviously alleviate the long-term liver toxicity caused by tripterygium polyglycoside to a certain extent, with a better prophylactic effect.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2020 Type: Article