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Effect of Xiaoyaosan on Changes of JAK/STAT Pathway in Hypothalamic Arcuate Nuclei of Rats With Chronic Mild Unpredictable Stress / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 21-27, 2020.
Article in Chinese | WPRIM | ID: wpr-872754
ABSTRACT

Objective:

To observe the changes of leptin receptor-tyrosine kinase Janus2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway and the regulatory effect of Xiaoyaosan on the hypothalamic arcuate nuclei of rats with chronic mild unpredictable stress model (CUMS).

Method:

Sixty male sprague-dawley rats were randomly divided into normal group, model group, Xiaoyaosan group, and fluoxetine group. After one-week adaptive feeding, the rats in model group, Xiaoyaosan group and fluoxetine group were uesd to replicate the chronic psychological stress rat model through mild unpredictable stimulation. Meanwhile , they were simultaneously administered the corresponding drugs, Xiaoyaosan 19.27 g·kg-1·d-1, Fluoxetine 2 mg·kg-1·d-1 (based on the average adult body weight of 60 kg), the rats in the normal group and the model group were given the same volume of normal saline for 6 weeks. The body weightfood intakesucrose consumption ratios, and the experimental behavior in the open field test (OFT) of the groups were observed. Real-time fluorescence quantitative polymerase chain reactionReal-time PCR) and Western blot were used to detect the expressions of ob-R, JAK2, and STAT3 in the arcuate nucleus of rat hypothalamus.

Result:

Compared with the normal group, the body weight and food intake of the model group were significantly decreased (P<0.05, P<0.01), the sucrose consumption ratios , the total behavioral distance of the experimental field and the total distance of the central area were significantly reduced, the protein and mRNA expressions of ob-R, JAK2, STAT3 in the arcuate nucleus of hypothalamus in rats increased significantly (P<0.05, P<0.01). Compared with the model group, the body weight of Xiaoyaosan group increased significantly on the 7th, 14th, 21st and 28th days (P<0.05, P<0.01), the food intake of rats increased significantly on the 21st and 35th days of the experiment (P<0.05), and the sucrose consumption ratios, the total distance of the experimental behavior in the open field test (OFT) and the total distance of the central area were significantly improved. Xiaoyaosan had a corresponding regulatory effect on the protein and mRNA expressions of ob-R, JAK2, STAT3 in the arcuate nucleus of hypothalamus in model rats (P<0.05, P<0.01).

Conclusion:

Xiaoyaosan regulates the body weightappetite, and energy metabolism of chronically mild and unpredictable stress rats, which may be related to the ob-R-JAK2/STAT3 pathway in the hypothalamic arcuate nucleus.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2020 Type: Article