Effect of Zuoguiwan on Serum Related Indexes and Pancreatic PDX-1 Expression in GDM RatsWANG Chao-qun1，2, WANG Yong-hui2, LIU Tao2, XU Kai-xia2, ZHAO Le2, ZHOU Ran2*（1.HuBei University of Traditional Chinese Medicine（TCM），Wuhan 430065，China；2.Shanxi University of TCM，Taiyuan 030024，China） / 中国实验方剂学杂志

ABSTRACT

Objective:To investigate the effect of maternal intervention of Zuoguiwan on the improvement of serum and pancreatic index in rats of gestational diabetes mellitus(GDM). Method:The GDM pregnant rats were replicated by monogamous cage and introneal injection of streptozotocin (STZ). The pregnant rats were randomly divided into normal group, model group, insulin group (20 U·kg-1), and low, medium and high-dose Zuoguiwan groups (2.5，5.0，10.0 g·kg-1). The insulin group was given hypodermic injection of portal winter insulin, all drug groups were respectively administrated with corrsponding drugs for 2 weeks, once a day. Meanwhile, control group and model group were given physiological saline by gavage. After that, the levels of serum insulin, fasting plasma glucose（FBG）, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-8 were measured by enzyme-linked immunosorbent assay (ELISA), protein and gene expression levels of Pancreas-duodenal homologous box factor-1 (PDX-1) were respectively detected by Western blot and real-time fluorescence quantitative polymerase chain reaction（Real-time PCR）. Result:Compared with normal group, FBG, TNF-α and IL-6 levels of pregnant rats in model group increased significantly (P<0.01), the level of IL-8 increased uniformly (P<0.05), and serum insulin level, protein and gene expression levels of PDX-1 decreased significantly (P<0.01). Compared with model group, FBG and serum IL-8 levels of pregnant rats in insulin group were significantly lower (P<0.01), while serum insulin level, protein and gene expression levels of PDX-1 increased significantly(P<0.01). FBG, IL-8, IL-6 and TNF-α levels in serum of pregnant rats in medium-dose Zuoguiwan group decreased obviously, meanwhile serum insulin level, protein and gene expression levels of PDX-1 increased significantly (P<0.01). Conclusion:Zuoguiwan may promote the secretion of insulin by reducing the serum inflammatory factors in GDM rats, and at the same time up-regulate the expression of PDX-1 protein and gene, so as to restrain insulin resistance, reduce the damage of pancreatic cells and improve the blood glucose of rats.