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Effect of Different Concentrations of Astragali Radix Containing Serum on CYP24A1,CYP27B1 mRNA and Protein in Osteogenic Differentiation of Aging Bone Marrow Mesenchymal Stem Cells / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 49-55, 2020.
Article in Chinese | WPRIM | ID: wpr-872919
ABSTRACT

Objective:

To investigate the effects of different concentrations of Astragali Radix containing serum on the expression of 24-hydroxylaseCYP24A1),1α-OHase(CYP27B1mRNA and protein in rat bone marrow mesenchymal stem cells (BMSCs), and to explore the mechanism of primary osteoporosis (OP).

Method:

The experiment was divided into 6 groups,like normal group, model group, low ,middle and high dose group of Astragali Radix containing serum(20%,40%,60%),Vitamin D group. Cell proliferation toxicity assay(CCK-8) was used to detect the effect of different concentrations of Astragali Radix containing serum on survival rate of aging BMSCs.Real-time quantitative PCRReal-time PCR) and Western blot was used to detect the expression of CYP24A1 CYP27B1 mRNA and protein in senile BMSCs osteogenic differentiation cells by different concentrations of Astragali Radix containing serum.

Result:

Compared with normal group, the proliferation and survival rate of BMSCs osteoblasts induced by D-galactose in model group was significantly lower than that in normal group (P<0.01). Compared with model group, medium and high dose groups and Vitamin D group could improve the proliferation and differentiation of aging BMSCs into osteoblasts in different degrees(P<0.01). The relative expression of CYP27B1 mRNA and protein in model group was significantly lower than that in normal group, while the relative expression of CYP24A1 mRNA and protein in model group was significantly higher than that in normal group. Compared with model group, high dose Astragali Radix containing serum group could increase the relative expression of CYP27B1 mRNA and protein, and decrease the relative expression of CYP24A1 mRNA and protein in a dose-dependent manner(P<0.01).

Conclusion:

The mechanism of different concentrations of Astragali Radix containing serum in the treatment of osteoporosis may be related to the regulation of CYP24A1CYP27B1 mRNA and protein in the osteogenic differentiation of aging BMSCs.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2020 Type: Article