Identification of Multi-source Ethnodrug Rodgersiae Rhizoma by DNA Barcoding Technology / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
;
(24): 142-150, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-872932
ABSTRACT
Objective:
Five popular DNA barcoding sequences,namely ITS,ITS2,rbcL,matK and psbA-trnH,were employed to evaluate the identification efficiency of multi-source ethnodrug Rodgersiae Rhizoma,and the most suitable sequence was then screened out.Method:
Efficiency of polymerase chain reaction(PCR) amplification,success rate of sequencing,intra- and inter-specific distances calculated by rank sum test,phylogenetic tree constructed with neighbor-joining(NJ) method and identification efficiency assessed by Blast 1 and NJ method were adopted in this study.Result:
Efficiency of PCR amplification and success rate of sequencing for ITS,ITS2,psbA-trnH,rbcL and matK were 100%,96.61% ,100%,98.31%,100%,100%,100%,100%,98.31% and 98.31%,respectively. Intra- and inter-specific genetic distances and identification achievement rate for psbA-trnH were the highest among the five candidate sequences. Besides,the average coalescent depth was less than the smallest interspecific distance for psbA-trnH. Phylogenetic tree also illustrated that Rodgersiae Rhizoma could be distinguished based on psbA-trnH.Conclusion:
According to the findings,psbA-trnH was superior to other DNA barcodes. Therefore, psbA-trnH was recommended as the ideal DNA barcode for the identification of multi-source ethnodrug Rodgersiae Rhizoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Experimental Traditional Medical Formulae
Year:
2020
Type:
Article
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