Investigation of Adsorption Properties of Five Macroporous Resins for Total Flavonoids in Epimedii Folium / 中国实验方剂学杂志

ABSTRACT

Objective:To compare the adsorption characteristics of different macroporous adsorption resins for the total flavonoids in Epimedii Folium, clarify the adsorption mechanism, and screen the optimal resin for the purification of total flavonoids in Epimedii Folium. Method:Taking the adsorption and desorption capacities of the total flavonoids in Epimedii Folium and five representative flavonoids (epimedin A, epimendin B, epimendin C, icariin, baohuoside Ⅰ) as indexes, static adsorption and dynamic adsorption experiments were conducted to compare the adsorption characteristics of five macroporous including HPD100, HPD600, AB-8, X-5 and D101.The adsorption kinetics of the selected resin was studied by using the pseudo-first-order, pseudo-second-order kinetic models and intraparticle diffusion model, and the thermodynamic process was analyzed by using the Langmuir and Freundlich isothermal adsorption models, which explored the adsorption mechanism of resin from the perspective of physical chemistry. Result:HPD100 macroporous resin had a better adsorption and desorption properties than the others. The adsorption process of HPD100 macroporous resin for total flavonoids in Epimedii Folium and five representative flavonoids conformed to the pseudo-second-order kinetic model. The adsorption thermodynamic process of HPD100 resin for total flavonoids of Epimedii Folium conformed to the Freundlich model, and for the sum of five representative flavonoids conformed to the Langmuir model. The adsorption process of HPD100 resin for total flavonoids in Epimedii Folium was the exothermic process dominated by physical adsorption, and the optimal adsorption temperature was 25 ℃. Conclusion:HPD100 macroporous resin has large adsorption capacity, easy desorption and clear adsorption mechanism, it is suitable for isolation and purification of total flavonoids in Epimedii Folium.