UPLC Fingerprint of Notopterygh Rhizoma et Radix Pieces with Different Packaging and Storage Treatments / 中国实验方剂学杂志

ABSTRACT

Objective:Ultra performance liquid chromatography (UPLC) fingerprint was used to evaluate the storage effect of different packaging and storage conditions on Notopterygh Rhizoma et Radix pieces. Method:UPLC fingerprints of Notopterygh Rhizoma et Radix pieces with different storage time and packaging conditions were established under following chromatographic conditions Waters UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm), mobile phase 0.3%phosphoric acid solution(A)-acetonitrile (B) for gradient elution (0-4 min, 90%-47%A; 4-8 min, 47%A, ; 8-12 min, 47%-20%A; 12-16 min, 20%A; 16-18 min, 20%-90%A), flow rate of 0.2 mL·min-1, column temperature at 35 ℃, detection wavelength of 246 nm, and injection volume of 1.0 μL. The fingerprints were evaluated in terms of similarity, cluster analysis and principal component analysis. Result:The similarity evaluation showed that the UPLC fingerprint patterns of Notopterygh Rhizoma et Radix pieces with different storage time and different packaging and storage treatments were basically the same, and the similarity was above 0.95.Systematic clustering shows that Notopterygh Rhizoma et Radix pieces packaged in plastic bags, stored under light and shade and the initial live-action pieces were clustered into one group. Principal component analysis showed that Notopterygh Rhizoma et Radix pieces packaged in plastic bags, stored under light and shade had the highest comprehensive scores. Conclusion:Storage time, packaging material and storage temperature will not cause the increase or decrease of internal components of Notopterygh Rhizoma et Radix, but only affect the content of specific components. Plastic bag packaging, light and cool storage conditions are more suitable for the preservation of non-volatile oil components of Notopterygh Rhizoma et Radix.