Chemical Constituents of Corydalis impatiens and Their Inhibitory Effect on Proliferation of Hepatoma Cells / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
;
(24): 163-168, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-873035
ABSTRACT
Objective:
To systematically investigate the chemical constituents of 90% ethanol extract of Corydalis impatiens,and evaluate their inhibitory effect on cell proliferation in vitro.Method:
The chemical constituents were isolated and purified by silica gel,Sephadex LH-20,ODS column chromatography and semi-preparative high performance liquid chromatography (HPLC).the structures were identified by spectroscopy methods such as NMR and MS,as well as analysis of physicochemical properties and/or comparison with literature data,and the inhibitory activities of 13 compounds on HepG2 and SMMC-7721 cells were measured by methyl thiazolyl tetrazolium (MTT) method.Result:
Thirteen known compounds were isolated and identified from 90% ethanol extract of Corydalis impatiens as ethyl-5-hydroxy-2-pyridinecarboxylate(1),coryhumolide(2),3,4-cis-3,4-dihydroxy-β-ionone(3),megastigmane(4),9-hydroxy-4,7-megastigmadien-3-one(5),blumenol A(6),indole-3-carboxy acid(7),1-methyl-[1,2,4]triazolo[4,3-b][1,2,4]triazin-7-one (8),adenine(9),nicotinamide(10),2-hydroxymethyl-5-pyridinol(11),adenosine(12), and β-daucosterol(13). Cytotoxicities assay showed that the IC50 value of compound 3 for the hepatic cell line HepG2 was 24.7 μmol·L-1 (positive control drug cisplatin:4.8 μmol·L-1),and IC50 value of compound 4 for the hepatic cell line SMMC-7721 was 13.8 μmol·L-1(positive control drug cisplatin:5.4 μmol·L-1).Conclusion:
Compound 1 was a new natural compound,compounds 3-8 were obtained from genus Corydalis for the first time,and compounds 2,9-12 were isolated from this plant for the first time. Compound 3 exhibited weak inhibitory effect on hepatic cell line HepG2,and compound 4 exhibited moderate inhibitory effect on hepatic cell line SMMC-7721.The other compounds rest of ones exhibited no obvious inhibitory effect on hepatic cell line HepG2 or SMMC-7721.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Experimental Traditional Medical Formulae
Year:
2020
Type:
Article
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