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Cluster Analysis of Ziziphora bungeana from Different Geographical Populations Based on ITS2 and psbA-trnH Sequences / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 175-182, 2020.
Article in Chinese | WPRIM | ID: wpr-873070
ABSTRACT

Objective:

To analyze the internal transcribed spacer(ITS)2 and psbA-trnH sequences of Ziziphora bungeana in 18 different geographic populations,in order to provide reference about evaluation of germplasm resources and analysis of genetic diversity of medicinal plants.

Method:

Genomic DNAs of the Z. bungeana were extracted by kit method. Polymerase chain reactionPCR)was used to amplify ITS2 and psbA-trnH interstitial region sequences,bidirectional sequencing,splicing,and constructing Neighbor-joining(NJ) based on Kimura 2-parameter(K2P) model.

Result:

All of sequences of ITS2 and psbA-trnH of Z. bungeana in different geographic populations showed intraspecific variations. The average ITS2 sequence length of Z. bungeana was 236 bp,9 haplotypes were detected,and the genetic distance was 0-0.022. Z. bungeana of different geographical groups gathered into two branches,10 geographic populations,including XTH3,XTH6 and XTH9,were considered as one branch,while 8 geographic populations, including XTH4,XTH5 and XTH10,were the other branch. In addition to XTH6 that lacked 6 in bp psbA-trnH sequence,all of the other geographic populations had a 355 bp sequence of psbA-trnH,4 haplotypes were detected,and the genetic distance was 0-0.023. 12 geographic populations,such as XTH1,XTH3,XTH4,gathered into one branch,while XTH14,XTH17 and XTH18 gathered into the other branch. NJ tree based on ITS2+psbA-trnH combination sequence showed that Z. bungeana of different geographical populations could be divided into two branches,with 12 geographical populations,like XTH11,XTH12,XTH16 as one branch, and XTH14,XTH17 and XTH18 as the other branch.

Conclusion:

Near or similar geographical locations of different geographical populations implies relatively short genetic distance and relatively close genetic relationship,which indicates that genetic relationship and genetic diversity of Z. bungeana in different geographical populations are related to geographical locations.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2020 Type: Article