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Molecular Methods for Studying the Human Microbiota
Article in Korean | WPRIM | ID: wpr-87589
Responsible library: WPRO
ABSTRACT
Vast array of microbes colonize to each anatomical environment of human body. Culture based methods are important in investigating the microbial structure, but they are extremely biased in their evaluation of microbial diversity by selecting particular population of microbiota. Recent advance in molecular technology has allowed sophisticated analysis of complex human microbiota by culture-independent methods. Here, we will discuss features of tools for human microbiota studies including Roche-454 and Illumina platform. We will also briefly discuss features of some strategies that are commonly applied to these platforms including 16S rRNA targeting and shotgun sequencing. New platforms such as PacBio and Oxford Nanopore are also introduced.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Humans / Bias / Colon / Human Body / Metagenome / Nanopores Language: Korean Journal: Journal of Bacteriology and Virology Year: 2013 Type: Article
Full text: Available Index: WPRIM (Western Pacific) Main subject: Humans / Bias / Colon / Human Body / Metagenome / Nanopores Language: Korean Journal: Journal of Bacteriology and Virology Year: 2013 Type: Article