Read-through Mutation in the Coat Protein ORF Suppresses Turnip Yellow Mosaic Virus Subgenomic RNA Accumulation
Journal of Bacteriology and Virology
;
: 54-63, 2013.
Article
in English
| WPRIM
| ID: wpr-87591
ABSTRACT
We have previously observed that a sequence in coat protein (CP) ORF of Turnip yellow mosaic virus (TYMV) is required for efficient replication of the virus. The sequence was predicted to take a stem-loop structure, thus termed SL2. While examining various SL2 mutants, we observed that all the modifications resulting in extension of translation beyond the CP ORF significantly suppressed subgenomic RNA accumulation. The genomic RNA level, in contrast, was not affected. Introduction of an in-frame stop codon in the CP ORF of these constructs restored the level of subgenomic RNA. Overall, the results suggest that the read-through makes the subgenomic RNA unstable.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Viruses
/
RNA
/
Tymovirus
/
Codon, Terminator
/
Brassica napus
/
Ecthyma, Contagious
Limits:
Animals
Language:
English
Journal:
Journal of Bacteriology and Virology
Year:
2013
Type:
Article
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