All-trans retinoic acid induces endoplasmic reticulum stress signaling pathway in ARPE-19 cells / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 963-968, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-876736
ABSTRACT
@#AIM:
To investigate the endoplasmic reticulum stress(ERS)induced by all-trans retinoic acid(ATRA)in ARPE-19 cells.<p>METHODS:
Immunofluorescence, real-time quantitative polymerase chain reaction and Western blot were used to detect the protein and mRNA expression of related signal pathways during the process of endoplasmic reticulum stress response induced by ATRA in ARPE-19 cells.<p>RESULTS:
With the accumulation of ATRA concentration, the protein and mRNA levels of endoplasmic reticulum stress response marker proteins chop and BiP were significantly increased(<i>P</i><0.001); in the downstream signaling pathways, perk, eIF2 α, ATF4, IRE1 α and XBP1 were up-regulated(<i>P</i><0.001), while the expression of ATF6 did not change(<i>P</i>>0.05).<p>CONCLUSION:
Over accumulation of ATRA induces ERS in ARPE-19 cells and activates PERK-EIF2 α-ATF4 and IRE1 α-XBP1 signaling pathways
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Eye Science
Year:
2021
Type:
Article
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