Study on mechanism of sodium arsenite-induced lung injury in male rats / 中国职业医学

ABSTRACT

OBJECTIVE: To observe the effects of lung injury caused by sodium arsenite in male rats,and to explore its cell senescence mechanism. METHODS: Specific pathogen free healthy adult male Wistar rats were randomly divided into 4groups with 8 rats in each group a control group( given ultrapure water) and low-,medium- and high-sodium arsenite dose groups( 10,100 and 1 000 μg / L sodium arsenite in drinking water,respectively). The rats were euthanized after 4weeks of treatment. The pathologic changes in the lung were examined and the levels of interleukin( IL)-1β,IL-6,IL-10 and β-galactosidase( β-Gal) in broncho-alveolar lavage fluid( BALF) were determined by enzyme-linked immunosorbent assay. RESULTS: The lung tissue of the 3 treatment groups showed early inflammatory pathological changes. With the increase of dose,lung histopathology gradually showed the alveolar interval widened,infiltrating with protein-rich fluid and a large number of inflammatory cells. The levels of IL-1β,IL-6 and β-Gal in BALF of the medium- and high-dose groups were higher than those of the control group( P < 0. 05). The levels of IL-10 in BALF of the 3 treatment groups were lower than that of the control group( P < 0. 05). The levels of β-Gal and IL-1β in BALF of rats as well as with IL-6 was positively correlated [the correlation coefficient( r) were 0. 691 and 0. 410 respectively,P < 0. 05]. The levels of β-Gal and IL-10 were not correlated( r =- 0. 117,P > 0. 05). CONCLUSION: Sodium arsenite in drinking can result in inflammatory injury in lung in male rats. The mechanism may be related to the cell senescense that activates the inflammatory cascade.