Detecting chlorpyrifos and its metabolitein plasma by UPLC / 中国职业医学

ABSTRACT

OBJECTIVE: To establish a simple and sensitive method for simultaneous detection of chlorpyrifos( CPF),and its metabolite 3,5,6-trichloro-2-pyridinol( TCP) in plasma samples by ultra performance liquid chromatography( UPLC).METHODS: The 0. 5 m L of blood sample was extracted by ethyl acetate,then separated by C18 column using acetonitrile /water as a mobile phase and detected by diode-array detector under the ultraviolet spectrum of 298 nm( 0. 0-2. 0 min) and289 nm( 2. 0-5. 0 min) through UPLC. RESULTS: The method showed that a good linear range was 1. 00-50. 00 mg / L of both CPF and TCP,and the correlation coefficients was 0. 999 9. The limit of detection was 0. 30 mg / L and the lower limits of quantitation was 1. 00 mg / L of both CPF and TCP. The recovery rates of CPF and TCP were 87. 20 %-103. 08 %and 86. 20 %-99. 28 %,respectively. The within-run relative standard deviation( RSD) of CPF and TCP was 5. 28 %-6. 17 % and 2. 32 %-4. 43 %,and the between-run RSD was 6. 62 %-7. 53 % and 3. 55 %-5. 24 %. Samples can be kept in4 degrees Celsius refrigerator for 7 days. CONCLUSION:s The proposed method is simple,reliable and sensitive. It can be applied for simultaneous detection of CPF and its metabolite TCP in plasma samples.