Serum microRNA expression profiling revealing potential diagnostic biomarkers for lung adenocarcinoma / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2532-2542, 2020.
Article
in English
| WPRIM
| ID: wpr-877846
ABSTRACT
BACKGROUND@#Recent studies have demonstrated that microRNAs (miRNAs) in the blood circulation can serve as promising diagnostic markers for cancers. This four-stage study aimed at finding serum miRNAs as potential biomarkers for lung adenocarcinoma (LA) diagnosis.@*METHODS@#The study was carried out between 2016 and 2017. The Exiqon miRNA qPCR panel (3 LA vs. 1 normal control [NC] pooled serum samples) was used for initial screening to acquire miRNA profiles. Thirty-five dysregulated miRNAs were further evaluated in the training (24 LA vs. 24 NCs) and testing stages (110 LA vs. 110 NCs) using quantitative real-time polymerase chain reaction assays.@*RESULTS@#Four serum miRNAs (miR-133a-3p, miR-584-5p, miR-10b-5p, and miR-221-3p) were significantly overexpressed in LA patients compared with NCs. The diagnostic value of the four-miRNA panel was validated by an external cohort (36 LA vs. 36 NCs). The areas under the receiver operating characteristic curve of the four-miRNA panel in the training, testing, and external validation stages were 0.734, 0.803, and 0.894 respectively. Meanwhile, the expression level of miR-221-3p was much higher in LA tumor samples than that in the adjacent normal tissues (19 LA vs. 19 NCs). The expression level of miR-10b-5p was also elevated in the serum-derived exosomes samples (18 LA vs. 18 NCs). The expression of miR-133a-3p, miR-584-5p, and miR-10b-5p was significantly elevated in LA patients with epidermal growth factor receptor mutation compared with NCs.@*CONCLUSION@#The study established a four-miRNA signature in serum that could improve the diagnostic capability of LA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Biomarkers
/
Biomarkers, Tumor
/
ROC Curve
/
Gene Expression Profiling
/
MicroRNAs
/
Adenocarcinoma of Lung
/
Lung Neoplasms
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2020
Type:
Article
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