MicroRNA-135a in ABCA1-labeled Exosome is a Serum Biomarker Candidate for Alzheimer's Disease / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 19-28, 2021.
Article
in English
| WPRIM
| ID: wpr-878317
ABSTRACT
Objective@#In the present study, the ABCA1 was used as a label to capture specific exosomes, the level of ABCA1-labeled exosomal microRNA-135a (miR-135a) was evaluated for the diagnosis of Alzheimer's disease (AD), especially in patients with early stages of AD.@*Methods@#This is a preliminary research focused on the levels of ABCA1 in WBCs, RBCs, HT-22 cells, and neuron cells. The diagnostic value of ABCA1-labeled exosomal miR-135a was examined using the CSF and serum of APP/PS1 double transgenic mice, and 152 patients with SCD, 131 patients with MCI, 198 patients with DAT, and 30 control subjects.@*Results@#The level of ABCA1 exosomes harvested from HT-22 cells and neuron culture medium was significantly higher compared to that of RBCs and WBCs ( @*Conclusion@#This study outlines a method to capture specific exosomes and detect them using immunological methods, which is more efficient for early diagnosis of AD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Mice, Transgenic
/
Biomarkers
/
Cell Line
/
MicroRNAs
/
Erythrocytes
/
Exosomes
/
Alzheimer Disease
/
Cognitive Dysfunction
/
ATP Binding Cassette Transporter 1
/
Leukocytes
Type of study:
Screening study
Limits:
Aged80
/
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2021
Type:
Article
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