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Exploration of an Efficient Simultaneous Molecular Detection Method of HIV, HCV, and Syphilis from a Single Dried Blood Spot / 生物医学与环境科学(英文)
Article in English | WPRIM | ID: wpr-878357
Responsible library: WPRO
ABSTRACT
Objective@#The aim of the present study was to evaluate the performance of the simultaneous detection of HIV-1 RNA, HIV-1 DNA, and HCV RNA using one dried blood spot (DBS) as an alternative sample to plasma.@*Method@#A total of 571 paired DBS/plasma samples were collected from men who have sex with men (MSM) and injection drug users (IDUs), and serological and molecular assays were performed. Using plasma results as the reference standard, the performance of DBS tests for HIV-1 RNA, HIV-1 DNA, and HCV RNA was evaluated. Pearson's correlation coefficients and Bland-Altman analysis were performed to assess the correlation and concordance between DBS and plasma.@*Results@#Among paired plasma/DBS samples with detectable HIV-1 RNA and HCV RNA, five samples (5/32) were not detectable in DBS, while measurable HIV-1 RNA levels were present in plasma (1.44 to 3.99 log @*Conclusion@#The performance of the simultaneous detection of HIV-1 RNA, HIV-1 DNA, and HCV RNA using one DBS was acceptable. DBS, as an alternative sample to plasma, may be a viable option for the simultaneous detection of HIV-1 RNA, HIV-1 DNA, and HCV RNA in resource-limited settings or for individuals living in areas that are difficult to access.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Specimen Handling / Treponema pallidum / DNA, Viral / RNA, Viral / Syphilis / HIV Infections / Sensitivity and Specificity / HIV-1 / Hepatitis C / Hepacivirus Language: English Journal: Biomedical and Environmental Sciences Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Specimen Handling / Treponema pallidum / DNA, Viral / RNA, Viral / Syphilis / HIV Infections / Sensitivity and Specificity / HIV-1 / Hepatitis C / Hepacivirus Language: English Journal: Biomedical and Environmental Sciences Year: 2021 Type: Article