Using dynamic molecular switches for shikimic acid production in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2104-2112, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-878470
ABSTRACT
Shikimic acid is an intermediate metabolite in the synthesis of aromatic amino acids in Escherichia coli and a synthetic precursor of Tamiflu. The biosynthesis of shikimic acid requires blocking the downstream shikimic acid consuming pathway that leads to inefficient production and cell growth inhibition. In this study, a dynamic molecular switch was constructed by using growth phase-dependent promoters and degrons. This dynamic molecular switch was used to uncouple cell growth from shikimic acid synthesis, resulting in the production of 14.33 g/L shikimic acid after 72 h fermentation. These results show that the dynamic molecular switch could redirect the carbon flux by regulating the abundance of target enzymes, for better production.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Shikimic Acid
/
Industrial Microbiology
/
Escherichia coli Proteins
/
Escherichia coli
/
Metabolic Engineering
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2020
Type:
Article
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