Construction of CAR-T cells targeting CS1 and analysis of their antitumor activity in vitro / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2162-2170, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-878475
ABSTRACT
We constructed the CS1-targeted second- and third-generation CAR-T cells with genetic engineered 4-1BB or/and ICOS as a costimulatory signaling molecule by use of lentiviral platform. The CS1-targeted second-generation CAR-T cells with ICOS or 4-1BB had similar anti-neoplastic activity. When effector/target ratio was 11, the CAR-T cells with ICOS showed better killing effect on IM9-lucgfp cells than those with 4-1BB. However, The CS1-targeted third-generation CAR-T cells exihibited lower cytolytic capacity against IM9-lucgfp cells than the CS1-targeted second-generation CAR-T cells when the ratio of effector/target was 11, 21 or 51. When the ratio of effector/target was 101, the killing efficacy of both the second- and third-generation CAR-T cells against IM9-lucgfp cells was more than 85%, significantly higher than that of the control T cells. Taken together, both the CS1-targeted second- and third-generation CAR-T cells with ICOS or/and 4-1BB could efficiently kill CS1-positive multiple myeloma cells, but the CS1-targeted second-generation CAR-T cells had more potent killing effect on CS1-positive multiple myeloma cells than the CS1-targeted third-generation CAR-T cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
T-Lymphocytes
/
Signal Transduction
/
Genetic Engineering
/
Xenograft Model Antitumor Assays
/
Cell Line, Tumor
/
4-1BB Ligand
/
Inducible T-Cell Co-Stimulator Protein
/
Multiple Myeloma
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2020
Type:
Article
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