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Identifying protein epitopes recognized by monoclonal antibodies / 生物工程学报
Chinese Journal of Biotechnology ; (12): 2443-2450, 2020.
Article in Chinese | WPRIM | ID: wpr-878500
ABSTRACT
To establish a method for identifying protein epitopes recognized by therapeutic monoclonal antibodies, the programmed death receptor-1 (PD-1) was selected as the target protein. Based on the alanine scanning strategy, a rapid expression method of antigen mutants combining site-directed mutagenesis with mammalian cell expression system was established, the conditions for eukaryotic expression element amplification and cell transfection expression were established. 150 PD-1 protein mutants were co-expressed, and the binding ability of these mutants to anti-PD-1 antibody Pembrolizumab was identified. The epitopes of Pembrolizumab were determined based on the binding ability of protein mutants to antibodies and combined with protein structure analysis, which was highly consistent with the reported crystal structure-based epitopes, indicating that this method is simple and accurate and can be used for epitope mapping of therapeutic monoclonal antibodies.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Epitope Mapping / Antibodies, Monoclonal / Epitopes / Antigens Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Epitope Mapping / Antibodies, Monoclonal / Epitopes / Antigens Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2020 Type: Article