Challenges and considerations on quality control and evaluation of pathogen metagenomic next-generation sequencing / 生物工程学报
Chinese Journal of Biotechnology
; (12): 2598-2609, 2020.
Article
in Zh
| WPRIM
| ID: wpr-878514
Responsible library:
WPRO
ABSTRACT
Metagenomic next-generation sequencing (mNGS) could be used for pathogen detection from nearly all types of clinical samples. Especially, the unique diagnostic capability of pathogen mNGS detecting unknown causative agent of infectious diseases makes this method become an importation complement and irreplaceable component for conventional routine laboratory test. However, the complexity of the testing process, the rapid product update, and the insufficiency in quality control and evaluation methods that all make clinical transformation, industry development, and regulation of this technology full of challenge and uncertainty. This review briefly introduces the technical advantages and challenges, and describes the general workflow and quality control steps in details. Finally, it focuses on current considerations regarding quality evaluation methods and standards for pathogen mNGS.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Quality Control
/
Communicable Diseases
/
Metagenome
/
Metagenomics
/
High-Throughput Nucleotide Sequencing
Type of study:
Guideline
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2020
Type:
Article