Advances in adaptive laboratory evolutionary engineering to microbial breeding / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 130-141, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-878548
ABSTRACT
In recent years, adaptive laboratory evolution (ALE) has emerged as a powerful tool for basic research in microbiology (e.g., molecular mechanisms of microbial evolution) and efforts on evolutionary engineering of microbial strains (e.g., accelerated evolution of industrial strains by bringing beneficial mutations). The ongoing rapid development of next-generation sequencing platforms has provided novel insights into growth kinetics and metabolism of microbes, and thus led to great advances of this technique. In this review, we summarize recent advances in the applications of long-term and short-term ALE techniques mainly for microbial strain engineering, and different modes of ALE are also introduced. Furthermore, we discuss the current limitations of ALE and potential solutions. We believe that the information reviewed here will make a significant contribution to further advancement of ALE.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
High-Throughput Nucleotide Sequencing
/
Laboratories
/
Mutation
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2021
Type:
Article
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