Progress in the construction and screening of random mutation library / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 163-177, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-878551
ABSTRACT
Directed evolution is a cyclic process that alternates between constructing different genes and screening functional gene variants. It has been widely used in optimization and analysis of DNA sequence, gene function and protein structure. It includes random gene libraries construction, gene expression in suitable hosts and mutant libraries screening. The key to construct gene library is the storage capacity and mutation diversity, to screen is high sensitivity and high throughput. This review discusses the latest advances in directed evolution. These new technologies greatly accelerate and simplify the traditional directional evolution process and promote the development of directed evolution.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Base Sequence
/
Proteins
/
Gene Library
/
Directed Molecular Evolution
/
Mutation
Type of study:
Controlled clinical trial
/
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2021
Type:
Article
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