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Progress in gene editing technologies for Saccharomyces cerevisiae / 生物工程学报
Chinese Journal of Biotechnology ; (12): 950-965, 2021.
Article in Chinese | WPRIM | ID: wpr-878606
ABSTRACT
Saccharomyces cerevisiae is one of the most important hosts in metabolic engineering. Advanced gene editing technology has been widely used in the design and construction of S. cerevisiae cell factories. With the rapid development of gene editing technology, early gene editing technologies based on recombinase and homologous recombination have been gradually replaced by new editing systems. In this review, the principle and application of gene editing technology in S. cerevisiae are summarized. Here, we first briefly describe the classical gene editing techniques of S. cerevisiae. Then elaborate the genome editing system of MegNs, ZFNs and TALENs based on endonuclease. The latest research progress is especially introduced and discussed, including the CRISPR/Cas system, multi-copy integration of heterologous metabolic pathways, and genome-scale gene editing. Finally, we envisage the application prospects and development directions of Saccharomyces cerevisiae gene editing technology.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Saccharomyces cerevisiae / Technology / Endonucleases / CRISPR-Cas Systems / Gene Editing Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Saccharomyces cerevisiae / Technology / Endonucleases / CRISPR-Cas Systems / Gene Editing Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article