Application of chronological lifespan in the construction of Escherichia coli cell factories / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1277-1286, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-878630
ABSTRACT
Microbial cell factories capable of producing valuable chemicals from renewable feedstocks provide a promising alternative towards sustainability. However, environmental stress remarkably affects the performance of microbial cell factories. By extending the chronological lifespan of microbial cells, the performance of microbial cell factories can be greatly improved. Firstly, an evaluation system for chronological lifespan and semi-chronological lifespan was established based on the changes in survival rates. Secondly, the addition of anti-aging drugs such as cysteine, carnosine, aminoguanidine and glucosamine increased the chronological lifespan of E. coli by 80%, 80%, 50% and 120%, respectively. Finally, we demonstrated that extending the chronological lifespan of E. coli increased the yield of metabolites produced by E. coli cell factories with endogenous (lactic acid and pyruvic acid) or exogenous (malic acid) metabolic pathway by 30.0%, 25.0%, and 27.0%, respectively. The strategy of extending chronological lifespan of E. coli provides a potential approach for enhancing the performance of microbial cell factories.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Lactic Acid
/
Escherichia coli
/
Metabolic Networks and Pathways
/
Metabolic Engineering
/
Longevity
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2021
Type:
Article
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