Application of SPR protein chip in screening for imported malaria / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1360-1367, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-878637
ABSTRACT
Imported malaria has become a major risk factor for malaria prevention and control in China. How to screen malaria quickly for people entering China is an urgent problem to be solved. Protein microarrays are widely used in high-throughput screening and diagnosis. In this study, surface plasmon resonance (SPR) technique for malaria detection was established by using the specific adsorption surface treated by polyethylene glycol polymer, and the malaria specific antigen HRP2 was used as capture probe. The optimal concentration of antigen, sensitivity and specificity of detection, as well as anti-interference ability of the chip were analyzed. The SPR protein chip was applied to detect specific antibodies of malignant malaria in serum with the advantage of label-free, instant and fast. Compared with fluorescence quantitative PCR, there were no significant difference in sensitivity and specificity between the two methods. This study lays a foundation for further development of protein microarray for malaria typing identification, and it is conducive to the rapid screening of malaria for people entering.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
China
/
Surface Plasmon Resonance
/
Protein Array Analysis
/
Malaria
/
Antibodies
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2021
Type:
Article
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