Thirty years development of ¹³C metabolic flux analysis: a review / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1510-1525, 2021.
Article
in Zh
| WPRIM
| ID: wpr-878652
Responsible library:
WPRO
ABSTRACT
¹³C metabolic flux analysis (¹³C-MFA) enables the precise quantification of intracellular metabolic reaction rates by analyzing the distribution of mass isotopomers of proteinogenic amino acids or intracellular metabolites through ¹³C labeling experiments. ¹³C-MFA has received much attention as it can help systematically understand cellular metabolic characteristics, guide metabolic engineering design and gain mechanistic insights into pathophysiology. This article reviews the advances of ¹³C-MFA in the past 30 years and discusses its potential and future perspective, with a focus on its application in industrial biotechnology and biomedicine.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Carbon Isotopes
/
Metabolic Engineering
/
Metabolic Flux Analysis
/
Amino Acids
/
Isotope Labeling
/
Models, Biological
Type of study:
Prognostic_studies
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2021
Type:
Article