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Comparative analysis of active components and transcriptome between autotetraploid and diploid of Dendrobium huoshanense / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-878828
Responsible library: WPRO
ABSTRACT
In this study, the roots, stems and leaves of diploid and autotetraploid Dendrobium huoshanense were used as materials to compare their contents of polysaccharides and alkaloids, and the transcriptome sequencing analysis was carried out. The results showed that the contents of polysaccharides and alkaloids in the roots, stems and leaves of tetraploid were 7.6%, 34.5%, 17.2%, 0.01%, 0.024% and 0.035% higher than those of diploid D. huoshanense, respectively. The contents of active components in different tissues were significantly different. There were 3 687 differentially expressed genes in diploid and tetraploid D. huoshanense, of which 2 346 genes were up-regulated and 1 341 down regulated. Go functional analysis showed that these genes were mainly involved in growth and development, stress resistance and other related functions. KEGG pathway analysis showed that most of the differential genes were concentrated in the processes of carbon metabolism, signal transduction, carbohydrate metabolism, amino acid metabolism and energy metabolism. The differential expression of key genes involved in the metabolism of polysaccharides, terpenes and polyketones, amino acid metabolism, hormone synthesis and signal transduction in diploid and tetraploid plants may be the main reason for the high energy content, the increase of active components and the growth potential of tetraploid plants.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Polysaccharides / Plant Roots / Dendrobium / Diploidy / Alkaloids / Transcriptome Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2020 Type: Article
Full text: Available Index: WPRIM (Western Pacific) Main subject: Polysaccharides / Plant Roots / Dendrobium / Diploidy / Alkaloids / Transcriptome Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2020 Type: Article