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Effect of swertiamarin, gentiopicrin and sweroside on cell apoptosis and expression of Bcl-2 in rheumatoid arthritis fibroblast-like synoviocytes / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 406-411, 2021.
Article in Chinese | WPRIM | ID: wpr-878988
ABSTRACT
The aim of this paper was to discuss the effect of swertiamarin, gentiopicrin and sweroside on rheumatoid arthritis fibroblast-like synoviocytes(RA-FLSs) and B-cell lymphoma-2(Bcl-2) and their mechanisms. ZINC database and RCSB PDB database were retrieved for 3 D chemical structures of swertiamarin, gentiopicrin and sweroside and 3 D target protein structures. AutoDock Mgltools 1.5.6, AutoDockVina 1.1.2 and pyMOL 2.2.0 were applied for molecular docking to analyze the relationship between Bcl-2(1 GJH) target protein and important ingredients. The cell apoptosis of RA-FLSs was tested by Annexin V-FITC. The Bcl-2 protein expression of RA-FLSs treated with different ingredients was tested by Western blot. The Bcl-2 mRNA expression of RA-FLSs treated with different ingredients was tested by RT-PCR. Swertiamarin, gentiopicrin and sweroside were docked well with Bcl-2(1 GJH). The binding energy of swertiamarin was-6.9 kcal·mol~(-1), the binding energy of gentiopicrin was-6.7 kcal·mol~(-1) and the binding energy of sweroside was-6.4 kcal·mol~(-1). Compared with the blank group, the Bcl-2 protein expression of each group were reduced, while that of the gentiopicrin group was the highest(P<0.01). Compared with the blank group, the Bcl-2 mRNA expression of each groups were reduced. Gentiopicrin can reduce the Bcl-2 protein expression and the Bcl-2 mRNA expression, so as to promote the RA-FLSs apoptosis.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Arthritis, Rheumatoid / Pyrones / Cells, Cultured / Apoptosis / Proto-Oncogene Proteins c-bcl-2 / Cell Proliferation / Iridoid Glucosides / Fibroblasts / Molecular Docking Simulation / Synoviocytes Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Arthritis, Rheumatoid / Pyrones / Cells, Cultured / Apoptosis / Proto-Oncogene Proteins c-bcl-2 / Cell Proliferation / Iridoid Glucosides / Fibroblasts / Molecular Docking Simulation / Synoviocytes Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2021 Type: Article