Protective effect of ginsenoside Rg_1 on hypoxia/reoxygenation injury and its mechanism / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1460-1466, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-879051
ABSTRACT
This project aimed to explore the protective effect of ginsenoside Rg_1 on hypoxia/reoxygenation(H/R)-induced H9 c2 cardiomyocyte injury and its underlying signaling pathway. The H/R model of H9 c2 cardiomyocytes was established and then the cells were divided into different treatment groups. CCK-8(cell counting kit-8) was used to detect the activity of cardiomyocytes; Brdu assay was used to detect the proliferation of H9 c2 cells; the caspase-3 activity was tested, and then the protein expression was assessed by Western blot. Flow cytometry was used to evaluate the apoptosis level of cardiomyocytes. Ginsenoside Rg_1 inhibited H/R-induced cardiomyocyte apoptosis and caspase-3 activity, promoted nuclear transcription of nuclear factor erythroid-2 related factor 2(Nrf2), and enhanced the expression of the downstream heme oxygenase-1(HO-1). Ginsenoside Rg_1 could increase Nrf2 nuclear transcription and HO-1 expression with the increase of concentration(10, 20, 40, 60 μmol·L~(-1)). However, the protective effect of ginsenoside Rg_1 on cardiomyocytes was significantly weakened after the transfection of Nrf2-siRNA. Ginsenoside Rg_1 could protect cardiomyocytes by activating the Nrf2/HO-1 pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Apoptosis
/
Myocytes, Cardiac
/
Ginsenosides
/
Heme Oxygenase-1
/
NF-E2-Related Factor 2
/
Hypoxia
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2021
Type:
Article
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