Rapid screening of single guide RNA targeting pig genome and the harvesting of monoclonal cells by microarray seal / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 111-121, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-879256
ABSTRACT
The emergence of regular short repetitive palindromic sequence clusters (CRISPR) and CRISPR- associated proteins 9 (Cas9) gene editing technology has greatly promoted the wide application of genetically modified pigs. Efficient single guide RNA (sgRNA) is the key to the success of gene editing using CRISPR/Cas9 technology. For large animals with a long reproductive cycle, such as pigs, it is necessary to screen out efficient sgRNA
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Clustered Regularly Interspaced Short Palindromic Repeats
/
CRISPR-Cas Systems
/
Gene Editing
Type of study:
Diagnostic study
/
Screening study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2021
Type:
Article
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