Your browser doesn't support javascript.
loading
Expression of Twist1, SIRT1, FGF2 and TGF-β3 genes and its regulatory effect on the proliferation of placenta, umbilical cord and dental pulp mesenchymal stem cells / 中华医学遗传学杂志
Article in Chinese | WPRIM | ID: wpr-879535
Responsible library: WPRO
ABSTRACT
OBJECTIVE@#To compare the mRNA level of cell proliferation-related genes Twist1, SIRT1, FGF2 and TGF-β3 in placenta mesenchymal stem cells (PA-MSCs), umbilical cord mensenchymals (UC-MSCs) and dental pulp mesenchymal stem cells (DP-MSCs).@*METHODS@#The morphology of various passages of PA-MSCs, UC-MSCs and DP-MSCs were observed by microscopy. Proliferation and promoting ability of the three cell lines were detected with the MTT method. Real-time PCR (RT-PCR) was used to determine the mRNA levels of Twist1, SIRT1, FGF2, TGF-β3.@*RESULTS@#The morphology of UC-MSCs and DP-MSCs was different from that of PA-MSCs. Proliferation ability and promoting ability of the PA-MSCs was superior to that of UC-MSCs and DP-MSCs. In PA-MSCs, expression level of Twist1 and TGF-β3 was the highest and FGF2 was the lowest. SIRT1 was highly expressed in UC-MSCs. With the cell subcultured, different expression levels of Twist1, SIRT1, FGF2, TGF-β3 was observed in PA-MSCs, UC-MSCs and DP-MSCs.@*CONCLUSION@#Up-regulated expression of the Twist1, SIRT1 and TGF-β3 genes can promote proliferation of PA-MSCs, UC-MSCs and DP-MSCs, whilst TGF-β3 may inhibit these. The regulatory effect of Twist1, SIRT1, FGF2 and TGF-β3 genes on PA-MSCs, UC-MSCs and DP-MSCs are different.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Placenta / Umbilical Cord / Female / Humans / Pregnancy / Nuclear Proteins / Cell Differentiation / Cells, Cultured / Fibroblast Growth Factor 2 / Dental Pulp Language: Chinese Journal: Chinese Journal of Medical Genetics Year: 2021 Type: Article
Full text: Available Index: WPRIM (Western Pacific) Main subject: Placenta / Umbilical Cord / Female / Humans / Pregnancy / Nuclear Proteins / Cell Differentiation / Cells, Cultured / Fibroblast Growth Factor 2 / Dental Pulp Language: Chinese Journal: Chinese Journal of Medical Genetics Year: 2021 Type: Article