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Effects and Mechanism of PARP Inhibitor Olaparib on the Expression of NKG2D Ligands in HL-60 Cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1826-1830, 2020.
Article in Chinese | WPRIM | ID: wpr-879978
ABSTRACT
OBJECTIVE@#To investigate the regulatory effects of Olaparib on natural killer cell activating receptor (NKG2D) ligands expression on human acute myeloid leukemia (AML) cell line HL-60, and to explore the molecular mechanism of Olaparib on HL-60 cells.@*METHODS@#After HL-60 cells in logarithmic growth phase were treated with Olaparib at different concentrations for different times (24, 48 h), the expression of NKG2D ligand on the surface of HL-60 cells was detected by flow cytometry. Western blot was used to dectect the expression of ERK expression in HL-60 cells. The killing effect of NK cells to HL-60 cells was detected by CFSE/PI method.@*RESULTS@#10 μmol/L Olaparib could upregulate the expression of NKG2D ligand on the surface of HL-60 cell at 24 and 48 hours, while 5 μmol/L Olaparib could induce up-regulation of the expression of ULBP-2 and ULBP-3 at 48 hours. Western blot analysis showed that ERK phosphorylation of HL-60 cells was enhanced after treating with Olaparib. The killing effect of NK cells to HL-60 cells could be enhanced by Olaparib, however, ERK inhibitor could suppress the killing effect of NK cells to HL-60 cells.@*CONCLUSION@#Olaparib can upregulate NKG2D ligands expression on the surface of HL-60 cells and enhance the cytotoxicity of NK cell to HL-60 cells. The mechanism may be related to Olaparib promoting ERK phosphorylation expression.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phthalazines / Piperazines / Histocompatibility Antigens Class I / HL-60 Cells / Cytotoxicity, Immunologic / Cell Line, Tumor / NK Cell Lectin-Like Receptor Subfamily K / Poly(ADP-ribose) Polymerase Inhibitors / Ligands Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phthalazines / Piperazines / Histocompatibility Antigens Class I / HL-60 Cells / Cytotoxicity, Immunologic / Cell Line, Tumor / NK Cell Lectin-Like Receptor Subfamily K / Poly(ADP-ribose) Polymerase Inhibitors / Ligands Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2020 Type: Article