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Effects of Soluble CD40 Ligand on Non-Hodgkin Lymphoma Cells and Its Relative Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1933-1938, 2020.
Article in Chinese | WPRIM | ID: wpr-879995
ABSTRACT
OBJECTIVE@#To explore the effect of soluble CD40 ligand (sCD40L) on the proliferation, apoptosis, and cell cycle of human non-Hodgkin lymphoma (NHL) cells, and analyze its possible mechanism.@*METHODS@#NHL CA46 cell and Raji cell were treated with different concentrations of sCD40L for 48 h, CCK-8 was used to detect the effect of sCD40L on cell proliferation in vitro, flow cytometry on apoptosis and cycle of NHL cells, and Western blot on the expression of PTEN, BCL-2, and BAX in NHL cells.@*RESULTS@#Compared with the control group, 4 and 8 μg/ml sCD40L could significantly inhibit the proliferation of lymphoma Raji cell and CA46 cell (P<0.05). The test results of flow cytometry showed that 4 μg/ml sCD40L could significantly promote the apoptosis of CA46 and Raji cells, and significantly inhibit the S phase proportions (P<0.05). Western blot results showed that sCD40L could promote the expression of PTEN and BAX, while inhibit the expression of BCL-2 (P<0.05).@*CONCLUSION@#sCD40L can promote the apoptosis and inhibit the proliferation of NHL cells through the PTEN signaling pathway.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Lymphoma, Non-Hodgkin / Family / Apoptosis / CD40 Ligand / Cell Line, Tumor / Cell Proliferation Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Lymphoma, Non-Hodgkin / Family / Apoptosis / CD40 Ligand / Cell Line, Tumor / Cell Proliferation Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2020 Type: Article