The Molecular Mechanism of LncRNA-CASC2/miR-155-5p/APC Axis Regulating the Progression of Non-Hodgkin Lymphoma / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1939-1945, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-879996
ABSTRACT
OBJECTIVE@#To investigate the effect of lncRNA-CASC2 (CASC2) /miR-155-5p/APC axis to the progression of non-Hodgikn lymphoma (NHL).@*METHODS@#The expression level of CASC2 and miR-155-5p in NHL cell lines were examined by qRT-PCR. Dual-luciferase reporter gene assay was used to verify the relationship between miR-155-5p, CASC2 and APC. The effects of CASC/miR-155-5p/APC axis to the proliferation, invasion and apoptosis of NK-92 cells were detected by MTT, Transwell assay and flow cytometry assay, respectively.@*RESULTS@#CASC2 was downregulated in NHL cell lines. Overexpression of CASC2 could inhibit the proliferation and invasion of NK-92 cells, and promote its apoptosis. Dual-luciferase reporter gene assay confirmed that there was a targeting relationship between miR-155-5p, CASC2 and APC. The restoration experiments proved that knockdown of both miR-155-5p and CASC2 or APC could restore the inhibitory effect of miR-155-5p silencing to the biological behavior of NK-92 cells.@*CONCLUSION@#Overexpression of CASC2 suppresses the proliferation and invasion of NK-92 cells, promote the apoptosis of NK-92 cells via targeting miR-155-5p and upregulating APC expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Lymphoma, Non-Hodgkin
/
Gene Expression Regulation, Neoplastic
/
Tumor Suppressor Proteins
/
MicroRNAs
/
Cell Line, Tumor
/
RNA, Long Noncoding
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2020
Type:
Article
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