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Establishment of Quantitative SPR Assay for Antibodies Against Human Platelet Antigen-1a / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 239-242, 2021.
Article in Chinese | WPRIM | ID: wpr-880061
ABSTRACT
OBJECTIVE@#To establish quantitative surface plasmon resonance (SPR) assay for antibodies against human platelet antigen-1a (HPA-1a).@*METHODS@#Recombinant protein was fixed on the chip surface by amino coupling method. SPR assay was used to detect the standard antibodies against HPA-1a at different conceatration. The optimal experimental parameters were determined, and standard curves were constructed with linear regression. Moreover, the sensitivity, specificity, accuracy and precision of the assay were evaluated.@*RESULTS@#The quantitative SPR assay for HPA-1a antibodies was established. The determination ranges were 0-20 IU, with accuracy (recovery rate) was 97.75%-103.08%. The intra-assay precision [coefficients of variation (CV)] was 3.53%-4.29%, and the inter-assay precision (CV) was 2.08%-4.40%. For specificity test, several kinds of monoclonal and human antibodies against platelet membrane protein were tested and no positive result was observed.@*CONCLUSION@#The established quantitative SPR assay for HPA-1a antibodies shows good sensitivity, specificity, accuracy and precision, and this rapid and simple method provides a new reference method for scientific research and clinical antibody detection.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Blood Platelets / Antigens, Human Platelet / Surface Plasmon Resonance / Isoantibodies Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Blood Platelets / Antigens, Human Platelet / Surface Plasmon Resonance / Isoantibodies Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2021 Type: Article