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Effect of Bmi-1 Expression on Chemotherapy Sensitivity in THP-1 Cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 363-368, 2021.
Article in Chinese | WPRIM | ID: wpr-880082
ABSTRACT
OBJECTIVE@#To investigate the effect of Bmi-1 expression on the chemosensitivity of THP-1 cells and its relative mechanism.@*METHODS@#The pGenesil-2-Bmi-1 1 siRNA, p-MSCV-Bmi-1 plasmid was transfected into THP-1 cells to reduce or increase the expression of Bmi-1. The expression of Bmi-1 mRNA and protein was verified by PCR and Western blot. The effect of camptothecin (CPT) on the proliferation and chemosensitivity of THP-1 cells affected by Bmi-1 gene were detected by MTT assay. The expression of DNA double-strand breaks marker-γ-H2AX was detected by immunofluorescence assay. Mitochondrial membrane potential and apoptosis were observed by flow cytometry. The expression of Cytochrome C, Caspase 3, Bax and BCL-2 was detected by Western blot.@*RESULTS@#Silencing Bmi-1 could inhibit proliferation and enhance the sensitivity of THP-1 cells to CPT, while overexpressed Bmi-1 could promote the cell proliferation and attenucate sensitivity of THP-1 cells to CPT. Silencing Bmi-1 could enhance CPT-induced DNA double-strand breaks, decrease mitochondrial membrane potential and promote CPT-induced apoptosis. While increasing Bmi-1 gene expression could attenuate CPT-induced DNA double-strand breaks, enhamce mitochondrial membrane potential and significantly reduce CPT-induced apoptosis of cells.@*CONCLUSION@#Bmi-1 expression could influence the sensitivity of THP-1 cells to CPT, and its relative mechanism may relate to DNA double-strand breaks and endogenous apoptotic pathways.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Camptothecin / Apoptosis / Cell Line, Tumor / Cell Proliferation / THP-1 Cells Language: Chinese Journal: Journal of Experimental Hematology Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Camptothecin / Apoptosis / Cell Line, Tumor / Cell Proliferation / THP-1 Cells Language: Chinese Journal: Journal of Experimental Hematology Year: 2021 Type: Article