Mechanisms for propofol in inhibiting the proliferation and invasion of glioma U87 cells and its effect on miR-134 expression / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 18-24, 2021.
Article
in English
| WPRIM
| ID: wpr-880617
ABSTRACT
OBJECTIVES@#To investigate the effects of propofol on the proliferation and invasion of glioma U87 cells and to explore the possible anti-tumor mechanisms.@*METHODS@#The glioma U87 cells was divided into a blank group, a positive control group, and the propofol groups (1.00, 2.00 or 5.00 mmol/L). Cell counting kit-8 (CCK-8) was used to detect cell proliferation; Transwell method was used to detect the effect of propofol on invasion and migration of U87 cells; real-time PCR was used to detect the expression of microRNA-134 (miR-134); Western blotting was used to detect the expression levels of reproduction-related protein Ki-67, invasion-related protein metalloproteinase-2 (MMP-2), metalloproteinase-9 (MMP-9) and phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) signaling pathway-related protein.@*RESULTS@#Compared with the blank group, the proliferation, invasion and migration capacity of U87 cells were reduced in the positive control group and the propofol groups after 48 hours (all @*CONCLUSIONS@#Propofol can decrease the proliferation rate, and the invasion and migration abilities of U87 cells, which may be achieved by up-regulation of miR-134 and suppression of PI3K/Akt signaling pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Propofol
/
Cell Movement
/
Phosphatidylinositol 3-Kinases
/
Matrix Metalloproteinase 2
/
MicroRNAs
/
Cell Line, Tumor
/
Cell Proliferation
/
Proto-Oncogene Proteins c-akt
/
Glioma
Limits:
Humans
Language:
English
Journal:
Journal of Central South University(Medical Sciences)
Year:
2021
Type:
Article
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