Your browser doesn't support javascript.
loading
Effect of small interfering RNA-mediated BIRC6 silencing on apoptosis and autophagy of renal cancer 786-O cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 1651-1655, 2020.
Article in Chinese | WPRIM | ID: wpr-880778
ABSTRACT
OBJECTIVE@#To study the expression of BIRC6 in renal cancer tissues and investigate the effect of BIRC6 silencing on apoptosis and autophagy of 786-O cells.@*METHODS@#Twenty surgical specimens of renal cancer tissues and adjacent renal tissues were collected from Meizhou People's Hospital between February, 2016 and December, 2018 for detection of BIRC6 protein expression using immunohistochemistry. Renal cancer 786-O cells were transfected with a control small interfering RNA (siRNA) or BIRC6 siRNA @*RESULTS@#The expression of BIRC6 protein was significantly higher in renal cancer tissues than in the adjacent renal tissues. Western blotting showed that siRNA-mediated silencing of BIRC6 significantly lowered the expression of BIRC6 in 786-O cells. In the cells with BIRC6 silencing, treatment with 12.5, 25, 50, 100 and 200 μg/mL 5-FU resulted in significantly higher proliferation inhibition rates than in the cells transfected with the control siRNA (@*CONCLUSIONS@#Interference of BIRC6 mediated by siRNA can inhibit autophagy and promote 5-FU-induced apoptosis to enhance the sensitivity of 786-O cells to 5-FU.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Autophagy / Apoptosis / RNA, Small Interfering / Cell Line, Tumor / Cell Proliferation / Inhibitor of Apoptosis Proteins / Kidney Neoplasms Limits: Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2020 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Autophagy / Apoptosis / RNA, Small Interfering / Cell Line, Tumor / Cell Proliferation / Inhibitor of Apoptosis Proteins / Kidney Neoplasms Limits: Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2020 Type: Article