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Association of JMJD3, MMP-2 and VEGF expressions with clinicopathological features of invasive ductal breast carcinoma / 南方医科大学学报
Article in Zh | WPRIM | ID: wpr-880780
Responsible library: WPRO
ABSTRACT
OBJECTIVE@#To examine the expressions of JMJD3, matrix metalloproteinase-2 (MMP-2) and vascular endothelial growth factor (VEGF) in invasive ductal breast carcinoma, their association with the clinicopathological features of the patients and the effect of JMJD3 overexpression on proliferation and MMP-2 and VEGF expressions in breast cancer cells.@*METHODS@#The protein and mRNA expressions of JMJD3, MMP-2, and VEGF in invasive ductal breast carcinoma and paired adjacent tissues were detected by immunohistochemistry and RT-PCR, respectively, and their correlation with the clinicopathological characteristics of the patients was analyzed. Kaplan-Meier survival analysis was used to evaluate the correlation of JMJD3, MMP-2 and VEGF expression levels with the survival of the patients. In breast cancer MDA-MB-231 cells transfected with a JMJD3-expression plasmid, the expression of Ki67 was examined immunohistochemically, the cell proliferation was assessed with CCK8 assay, and the mRNA expressions of MMP-2 and VEGF were detected with RT-PCR.@*RESULTS@#Breast cancer tissues had significantly lower JMJD3 expression and higher MMP-2 and VEGF expressions at both the mRNA and protein levels than the adjacent tissue (@*CONCLUSIONS@#The expressions of JMJD3, MMP-2 and VEGF in invasive ductal breast carcinoma are closely correlated to tumor proliferation, invasion, metastasis and prognosis and can be used for prognostic evaluation of breast cancer.
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Full text: 1 Index: WPRIM Main subject: Prognosis / Breast Neoplasms / Carcinoma, Ductal, Breast / Matrix Metalloproteinase 2 / Vascular Endothelial Growth Factor A / Jumonji Domain-Containing Histone Demethylases / Lymphatic Metastasis Type of study: Prognostic_studies Limits: Humans Language: Zh Journal: Journal of Southern Medical University Year: 2020 Type: Article
Full text: 1 Index: WPRIM Main subject: Prognosis / Breast Neoplasms / Carcinoma, Ductal, Breast / Matrix Metalloproteinase 2 / Vascular Endothelial Growth Factor A / Jumonji Domain-Containing Histone Demethylases / Lymphatic Metastasis Type of study: Prognostic_studies Limits: Humans Language: Zh Journal: Journal of Southern Medical University Year: 2020 Type: Article