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Palbociclib induces cell cycle arrest and senescence of human renal tubular epithelial cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 1784-1792, 2020.
Article in Chinese | WPRIM | ID: wpr-880803
ABSTRACT
OBJECTIVE@#To investigate the effect of palbociclib on cell cycle progression and proliferation of human renal tubular epithelial cells.@*METHODS@#Human renal tubular epithelial cell line HK-2 was treated with 1, 5, 10, and 20 μmol/L of palbociclib, and the changes in cell proliferation and viability were examined by cell counting and CCK8 assay. EDU staining was used to assess the proliferation of HK-2 cells following palbiciclib treatment at different concentrations for 5 days. The effect of palbociclib on cell cycle distribution of HK-2 cells was evaluated using flow cytometry. SA-β-Gal staining and C12FDG senescence staining were used to detect senescence phenotypes of HK-2 cells after palbociclib treatment at different concentrations for 5 days. The relative mRNA expression levels of P16, P21, and P53 and the genes associated with senescence-related secretion phenotypes were detected by RT-PCR, and the protein expressions of P16, P21 and P53 were detected by Western blotting.@*RESULTS@#Palbociclib inhibited HK-2 cell proliferation and induced cell cycle arrest in G1 phase. Compared with the control cells, HK-2 cells treated with high-dose (10 μmol/L) palbociclib exhibited significantly suppressed cell proliferation activity, and the inhibitory effect was the most obvious on day 5 (@*CONCLUSIONS@#Palbociclib induces HK-2 cell senescence by causing cell growth arrest and delaying cell cycle progression.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Piperazines / Pyridines / Cell Cycle / Tumor Suppressor Protein p53 / Cellular Senescence / Epithelial Cells / Cell Cycle Checkpoints Limits: Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Piperazines / Pyridines / Cell Cycle / Tumor Suppressor Protein p53 / Cellular Senescence / Epithelial Cells / Cell Cycle Checkpoints Limits: Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2020 Type: Article