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Ginsenoside 20(S)-Rg3 upregulates tumor suppressor VHL gene expression by suppressing DNMT3A-mediated promoter methylation in ovarian cancer cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 100-106, 2021.
Article in Chinese | WPRIM | ID: wpr-880834
ABSTRACT
OBJECTIVE@#To explore the mechanism by which ginsenoside 20(S)-Rg3 upregulates the expression of tumor suppressor von Hippel-Lindau (VHL) gene in ovarian cancer cells.@*METHODS@#Ovarian cancer cell line SKOV3 treated with 20(S)-Rg3 were examined for mRNA and protein levels of VHL, DNMT1, DNMT3A and DNMT3B by real-time PCR and Western blotting, respectively. The changes in VHL mRNA expression in SKOV3 cells in response to treatment with 5-Aza-CdR, a DNA methyltransferase inhibitor, were detected using real-time PCR. VHL gene promoter methylation was examined with methylation-specific PCR and VHL expression levels were determined with real-time PCR and Western blotting in non-treated or 20(S)-Rg3-treated SKOV3 cells and in 20(S)-Rg3-treated DNMT3A-overexpressing SKOV3 cells. VHL and DNMT3A protein levels were detected by immunohistochemistry in subcutaneous SKOV3 cell xenografts in nude mice.@*RESULTS@#Treatment of SKOV3 cells with 20(S)-Rg3 significantly upregulated VHL and downregulated DNMT3A expressions at both the mRNA and protein levels (@*CONCLUSIONS@#Ginsenoside 20(S)-Rg3 upregulates VHL expression in ovarian cancer cells by suppressing DNMT3A-mediated DNA methylation.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Ovarian Neoplasms / Gene Expression / Promoter Regions, Genetic / DNA Methylation / Ginsenosides / Cell Line, Tumor / Von Hippel-Lindau Tumor Suppressor Protein / Mice, Nude Limits: Animals / Female / Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Ovarian Neoplasms / Gene Expression / Promoter Regions, Genetic / DNA Methylation / Ginsenosides / Cell Line, Tumor / Von Hippel-Lindau Tumor Suppressor Protein / Mice, Nude Limits: Animals / Female / Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2021 Type: Article