Expression of NAD(P)H Oxidase Subunits and Their Contribution to Cardiovascular Damage in Aldosterone/Salt-Induced Hypertensive Rat
Journal of Korean Medical Science
;
: 1039-1045, 2008.
Article
in English
| WPRIM
| ID: wpr-8809
ABSTRACT
NAD(P)H oxidase plays an important role in hypertension and its complication in aldosterone-salt rat. We questioned whether NAD(P)H oxidase subunit expression and activity are modulated by aldosterone and whether this is associated with target- organ damage. Rats were infused with aldosterone (0.75 microgram/hr/day) for 6 weeks and were given 0.9% NaCl+/-losartan (30 mg/kg/day), spironolactone (200 mg/kg/ day), and apocynin (1.5 mM/L). Aldosterone-salt hypertension was prevented completely by spironolactone and modestly by losartan and apocynin. Aldosterone increased aortic NAD(P)H oxidase activity by 34% and spironolactone and losartan inhibited the activity. Aortic expression of the subunits p47(phox), gp91(phox), and p22(phox) increased in aldosterone-infused rats by 5.5, 4.7, and 3.2-fold, respectively, which was decreased completely by spironolactone and partially by losartan and apocynin. Therefore, the increased expression of NAD(P)H oxidase may contribute to cardiovascular damage in aldosterone-salt hypertension through the increased expression of each subunit.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Size
/
Aorta
/
Acetophenones
/
Spironolactone
/
Blood Pressure
/
RNA, Messenger
/
Sodium Chloride
/
Anti-Inflammatory Agents, Non-Steroidal
/
Rats, Sprague-Dawley
/
Superoxides
Limits:
Animals
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2008
Type:
Article
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