Effects of Esomeprazole on Pharmacokinetic Behavior of Sulfasalazine in Rats / 中国药房
China Pharmacy
;
(12): 1596-1601, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-881461
ABSTRACT
OBJECTIVE:To develop a metho d for determining the plasma concentration of sulfasalazine (SSZ)metabolite sulfapyridine(SP)in rats ,and to investigate the effects of esomeprazole (ESOM)on the pharmacokinetic behavior of SSZ in rats. METHODS:Male SD rats were randomly divided into SSZ group and SSZ+ESOM group ,with 6 rats in each group. SSZ+ESOM group were given Esomeprazole enteric-coated tablets [ 90 mg/(kg·d)] intragastrically for 14 days. On the 15th day ,the rats in 2 groups were given Sulfasalazine enteric coated tablets (90 mg/kg)intragastrically,and blood sample was collected from the inner canthus at 0.5,1,1.5,2,3,4,6,8,10,12,24,36,48,72 h after administration. After protein precipitation with methanol , using diazepam as internal standard ,Agilent XDR-C 18 column was adopted with methanol- 0.1% formic acid solution (gradient elution)as mobile phase. The concentration of SSZ metabolite SP in plasma was determined by LC-MS/MS. The pharmacokinetic parameters were calculated by using DAS 3.0.1 software and compared between 2 groups. RESULTS :The linear range of SP were 2-1 000 ng/mL. The methodology met the requirements of Chinese Pharmacopeia . There was no statistical significance in pharmacokinetic parameters of SP between 2 groups,such as AUC 0-t,tmax,t1/2z,cmax,MRT0-t(P>0.05). CONCLUSIONS :The established method is simple ,rapid and sensitive ;it can be used for the concentration determination of SSZ metabolite SP in plasma. ESOM has no significant effect on the pharmacokinetic behavior of SSZ in rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2021
Type:
Article
Similar
MEDLINE
...
LILACS
LIS