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Identification of Vibrio parahaemolyticus based on the concatenated DNA sequencing of 16S rRNA and gyrB gene / 上海预防医学
Shanghai Journal of Preventive Medicine ; (12): 372-376, 2021.
Article in Chinese | WPRIM | ID: wpr-881470
ABSTRACT

Objective:

To establish the concatenated DNA sequencing of 16S ribosomal RNA16S rRNA) and DNA gyrase subunit B (gyrB) gene, and provide evidence for the identification and classification of Vibrio parahaemolyticus (V. parahaemolyticus).

Methods:

Typical strains in the genus of Vibrio spp. was selected, such as V. parahaemolyticus, V. alginolyticus and other species for examination of 16S rRNA and gyrB gene as target. Primers were separately designed to amplify these two nucleotide fragments. Phylogenetic analysis was performed using the concatenated sequences.

Results:

The concatenated 16S rRNA+gyrB nucleotide sequence of V. parahaemolyticus formed a single cluster in the phylogenetic analysis, which identified the typical strains of Vibro spp. at the species level.

Conclusion:

In our study, an identification method of V. parahaemolyticus is established based on concatenated 16S rRNA+gyrB nucleotide sequencing. It can identify the strains of V. parahaemolyticus at the species level, which may be applied in phylogenetic analysis and contamination tracing of V. parahaemolyticus in food and drug control.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Shanghai Journal of Preventive Medicine Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Shanghai Journal of Preventive Medicine Year: 2021 Type: Article