Rapid screening of Salmonella by loop-mediated isothermal amplification combined with lateral flow dipstick / 上海预防医学
Shanghai Journal of Preventive Medicine
;
(12): 492-495, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-881493
ABSTRACT
Objective:
To establish a specific and sensitive method using loop-mediated isothermal amplification for rapid screening of Salmonella.Methods:
The invA gene sequence of Salmonella was downloaded from GenBank. After homology comparison with DNAMAN software, amplification primers were designed in the conserved region, and a LAMP-LFD detection method was established. The reaction system was optimized, and the specificity and sensitivity of the method were verified.Results:
The sensitivity of this method to detect Salmonella DNA was up to 1.0×101 copies/μL. The positive rate of anal swabs was the same as that of fluorescent PCR. Meanwhile, LAMP-LFD was easy to operate and did not need expensive instruments. The detection result could be obtained within 30 minutes.Conclusion:
The LAMP-LFD method established in this study is rapid, simple, sensitive and specific, which is suitable for rapid screening of Salmonella.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
Shanghai Journal of Preventive Medicine
Year:
2021
Type:
Article
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